Validation of a hepatitis B virus (HBV) Next Generation Sequencing (NGS) pipeline at Barts Health NHS Trust
نویسندگان
چکیده
منابع مشابه
Distribution of Hepatitis B Virus (HBV) Genotypes among HBV Carriers in Isparta
The aim of this study was to investigate the prevalence of hepatitis B virus (HBV) genotypes in Isparta, Southwest of Turkey, as well as the clinical features and transmission route for patients with HBV infections. Methods: Patients (n = 135) with HBV infection were included in the study. Epidemiological and clinical data were obtained. HBV genotypes were determined with a preS2 epitope ELISA ...
متن کاملIdentification of Drug Resistant Mutants of HBV (Hepatitis B Virus) by Direct Sequencing in Iranian Patients Treated with Lamivudine
Background and Aims: lamivudine is amongst the antiviral for drug chronic hepatitis B treatment. During therapy with lamivudine, variants may emerge with YMDD mutation in the reverse transcriptase (RT) region of polymerase gene. This mutation might have a role in drug resistant for HBV. Materials and Methods: HBV DNA extraction from serum sample of 88 patients, were subjected to nested PCR for ...
متن کاملBioruptor® NGS: Unbiased DNA shearing for Next-Generation Sequencing
Wouter Coppieters, PhD, and his colleagues operate the genomics core facility at the GIGA center, University of Liège (Belgium). The center routinely performs a variety of Next-Generation Sequencing (NGS) applications including de novo sequencing, whole genome and amplicon sequencing, and targeted resequencing on an Illumina GAIIx analyzer. Coppieters recognizes that optimal sequencing results ...
متن کاملNext Generation Sequencing (NGS) Technology in Oncology Drug Development
Overview of NGS technology In the decade since the completion of the human genome project, DNA sequencing technology has changed considerably. The commercial availability of the first massively parallel pyrosequencing platform in 2005 led to a new era of high-throughput genomic analysis, now referred to as next-generation sequencing (NGS). NGS technologies use a fundamentally different approach...
متن کاملMicrosatellites for Next-Generation Ecologists: A Post-Sequencing Bioinformatics Pipeline
Microsatellites are the markers of choice for a variety of population genetic studies. The recent advent of next-generation pyrosequencing has drastically accelerated microsatellite locus discovery by providing a greater amount of DNA sequencing reads at lower costs compared to other techniques. However, laboratory testing of PCR primers targeting potential microsatellite markers remains time c...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Access Microbiology
سال: 2019
ISSN: 2516-8290
DOI: 10.1099/acmi.ac2019.po0503